Sequencing:Article Title: Male-dependent resistance to Spiroplasma -induced cytoplasmic incompatibility
Article Snippet: Consensus sequences per Spiroplasma strain and gene were created with MEGA version 11 [ ] and BioEdit version 7.2.5 [ ] by manual analysis and editing.
Article Title: Helminth fauna of the monocled cobra (Naja kaouthia) from central Thailand: Community composition and taxonomic perspectives
Article Snippet: The obtained sequences were manually reviewed and edited using BioEdit version 7.2.5 (Hall, 1999).
Article Title: Are Babesia vogeli genotypes associated with Rhipicephalus sanguineus and Rhipicephalus linnaei distribution?
Article Snippet: Forward and reverse sequences were aligned and trimmed (error probability = 0.05) to generate consensus sequences using BioEdit version 7.2.5 (North Carolina State a Internal primers used for sequencing b Primers used on the screening PCR 18S rRNA Piro 0F (GCC AGT AGT CAT ATG CTT GTC TCA) ~1500 Denaturation at 95 °C for 5 min; 35 cycles at 94 °C for 1 min 30 s, 58 °C for 1 min, and 72 °C for 2 min; and extension at 72 °C for 10 min Kawabuchi et al. [47], Soares et al. [1], Mongruel et al. [48]Piro 6R (CTC CTT CCT YTA AGT GAT AAG GTT CAC) Piro 5.5Ra (CCT YTA AGT GAT AAG GTT CAC AAA ACT T) Bab 2fa (CCG TGC TAA TTG TAG GGC TAA TAC A) Bab 2f2a (CTT TGA GAA ATT AGA GTG TTT) Bab 2ra (TTC CTT AGA TGT GGT AGC CGT TTC) ITS1 F (GCT GCG TCC TTC ATC GTT GTG) ~300 Denaturation at 94 °C for 2 min; 30 cycles at 94 °C for 30 s, 60–66 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Bostrom et al. [49] R (CGA TCG AGT GAT CCG GTG AAT TA) ITS2 F (GTG AAC CTT ATC ACT TAA AGG) ~300 Denaturation at 96 °C for 3 min; 30 cycles at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 1 min 30 s; extension at 72 °C for 7 min Duarte et al. [50] R (CAA CTC CTC CAC GCA ATC G) hsp70 Bvog751Fb (CCG TAT CCC CAA GAT CCA GC) ~170 Denaturation at 95 °C for 5 min; 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; and final extension of 72 °C for 5 min This study Bvog926Rb (AGA GGG GAG CAA CGT CCA A) F1 (CAT GAA GCA CTG GCC HTT CAA) ~740 Denaturation at 95 °C for 5 min; 36 cycles at 95 °C for 15 s, 58.5–60 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Soares et al. [1] R1a (GCN CKG CTG ATG GTG GTG TTG TA) F2a (GGA TCA ACA AYG GMA AGA AC) ~720 R2 (GBA GGT TGT TGT CCT TVG TCA T) cox1 F (GGA AGT GGW ACW GGW TGG AC) ~900 Denaturation at 95 °C for 1 min; 35 cycles at 95 °C for 15 s, 45–57 °C for 30 s, and 72 °C for 1 min; and extension at 72 °C for 10 min Schreeg et al. [42] R (TTC GGT ATT GCA TGC CTT G) cox3 F (ACT GTC AGC TAA AAC GTA TC) ~700 Denaturation at 94 °C for 5 min; 40 cycles at 94 °C for 20 s, 55 °C for 30 s, and 68 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (ACA GGA TTA GAT ACC CTG G) cytb F (TTA GTG AAG GAA CTT GAC AGG T) ~900 Denaturation at 95 °C for 5 s; 35 cycles at 94 °C for 20 s, 58 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (CGG TTA ATC TTT CCT ATT CCT TAC G) University).
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis.
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi, and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: Beyond the known: Unraveling the existence of novel Trichinella genotypes (T14 and T15) in India and expanding the species complex
Article Snippet: A consensus sequence for each isolate was generated using BioEdit version 7.2.5 and subsequently submitted to the GenBank database, from which accession numbers were obtained ( ).
Article Title: Impact of Ozonated Water on the Fungal Colonies, Diversity and Fruit Quality of Grapevine in Northern Europe
Article Snippet: The sequences were edited using BioEdit version 7.2.5 [24].
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi , and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: PLOS Neglected Tropical Diseases
Article Snippet: A sequence identity 198 matrix was obtained with BioEdit version 7.2.5.
Generated:Article Title: Male-dependent resistance to Spiroplasma -induced cytoplasmic incompatibility
Article Snippet: Consensus sequences per Spiroplasma strain and gene were created with MEGA version 11 [ ] and BioEdit version 7.2.5 [ ] by manual analysis and editing.
Article Title: Helminth fauna of the monocled cobra (Naja kaouthia) from central Thailand: Community composition and taxonomic perspectives
Article Snippet: The obtained sequences were manually reviewed and edited using BioEdit version 7.2.5 (Hall, 1999).
Article Title: Are Babesia vogeli genotypes associated with Rhipicephalus sanguineus and Rhipicephalus linnaei distribution?
Article Snippet: Forward and reverse sequences were aligned and trimmed (error probability = 0.05) to generate consensus sequences using BioEdit version 7.2.5 (North Carolina State a Internal primers used for sequencing b Primers used on the screening PCR 18S rRNA Piro 0F (GCC AGT AGT CAT ATG CTT GTC TCA) ~1500 Denaturation at 95 °C for 5 min; 35 cycles at 94 °C for 1 min 30 s, 58 °C for 1 min, and 72 °C for 2 min; and extension at 72 °C for 10 min Kawabuchi et al. [47], Soares et al. [1], Mongruel et al. [48]Piro 6R (CTC CTT CCT YTA AGT GAT AAG GTT CAC) Piro 5.5Ra (CCT YTA AGT GAT AAG GTT CAC AAA ACT T) Bab 2fa (CCG TGC TAA TTG TAG GGC TAA TAC A) Bab 2f2a (CTT TGA GAA ATT AGA GTG TTT) Bab 2ra (TTC CTT AGA TGT GGT AGC CGT TTC) ITS1 F (GCT GCG TCC TTC ATC GTT GTG) ~300 Denaturation at 94 °C for 2 min; 30 cycles at 94 °C for 30 s, 60–66 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Bostrom et al. [49] R (CGA TCG AGT GAT CCG GTG AAT TA) ITS2 F (GTG AAC CTT ATC ACT TAA AGG) ~300 Denaturation at 96 °C for 3 min; 30 cycles at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 1 min 30 s; extension at 72 °C for 7 min Duarte et al. [50] R (CAA CTC CTC CAC GCA ATC G) hsp70 Bvog751Fb (CCG TAT CCC CAA GAT CCA GC) ~170 Denaturation at 95 °C for 5 min; 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; and final extension of 72 °C for 5 min This study Bvog926Rb (AGA GGG GAG CAA CGT CCA A) F1 (CAT GAA GCA CTG GCC HTT CAA) ~740 Denaturation at 95 °C for 5 min; 36 cycles at 95 °C for 15 s, 58.5–60 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Soares et al. [1] R1a (GCN CKG CTG ATG GTG GTG TTG TA) F2a (GGA TCA ACA AYG GMA AGA AC) ~720 R2 (GBA GGT TGT TGT CCT TVG TCA T) cox1 F (GGA AGT GGW ACW GGW TGG AC) ~900 Denaturation at 95 °C for 1 min; 35 cycles at 95 °C for 15 s, 45–57 °C for 30 s, and 72 °C for 1 min; and extension at 72 °C for 10 min Schreeg et al. [42] R (TTC GGT ATT GCA TGC CTT G) cox3 F (ACT GTC AGC TAA AAC GTA TC) ~700 Denaturation at 94 °C for 5 min; 40 cycles at 94 °C for 20 s, 55 °C for 30 s, and 68 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (ACA GGA TTA GAT ACC CTG G) cytb F (TTA GTG AAG GAA CTT GAC AGG T) ~900 Denaturation at 95 °C for 5 s; 35 cycles at 94 °C for 20 s, 58 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (CGG TTA ATC TTT CCT ATT CCT TAC G) University).
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis.
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi, and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: Beyond the known: Unraveling the existence of novel Trichinella genotypes (T14 and T15) in India and expanding the species complex
Article Snippet: A consensus sequence for each isolate was generated using BioEdit version 7.2.5 and subsequently submitted to the GenBank database, from which accession numbers were obtained ( ).
Article Title: Impact of Ozonated Water on the Fungal Colonies, Diversity and Fruit Quality of Grapevine in Northern Europe
Article Snippet: The sequences were edited using BioEdit version 7.2.5 [24].
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi , and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: PLOS Neglected Tropical Diseases
Article Snippet: A sequence identity 198 matrix was obtained with BioEdit version 7.2.5.
Polymerase Chain Reaction:Article Title: Male-dependent resistance to Spiroplasma -induced cytoplasmic incompatibility
Article Snippet: Consensus sequences per Spiroplasma strain and gene were created with MEGA version 11 [ ] and BioEdit version 7.2.5 [ ] by manual analysis and editing.
Article Title: Helminth fauna of the monocled cobra (Naja kaouthia) from central Thailand: Community composition and taxonomic perspectives
Article Snippet: The obtained sequences were manually reviewed and edited using BioEdit version 7.2.5 (Hall, 1999).
Article Title: Are Babesia vogeli genotypes associated with Rhipicephalus sanguineus and Rhipicephalus linnaei distribution?
Article Snippet: Forward and reverse sequences were aligned and trimmed (error probability = 0.05) to generate consensus sequences using BioEdit version 7.2.5 (North Carolina State a Internal primers used for sequencing b Primers used on the screening PCR 18S rRNA Piro 0F (GCC AGT AGT CAT ATG CTT GTC TCA) ~1500 Denaturation at 95 °C for 5 min; 35 cycles at 94 °C for 1 min 30 s, 58 °C for 1 min, and 72 °C for 2 min; and extension at 72 °C for 10 min Kawabuchi et al. [47], Soares et al. [1], Mongruel et al. [48]Piro 6R (CTC CTT CCT YTA AGT GAT AAG GTT CAC) Piro 5.5Ra (CCT YTA AGT GAT AAG GTT CAC AAA ACT T) Bab 2fa (CCG TGC TAA TTG TAG GGC TAA TAC A) Bab 2f2a (CTT TGA GAA ATT AGA GTG TTT) Bab 2ra (TTC CTT AGA TGT GGT AGC CGT TTC) ITS1 F (GCT GCG TCC TTC ATC GTT GTG) ~300 Denaturation at 94 °C for 2 min; 30 cycles at 94 °C for 30 s, 60–66 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Bostrom et al. [49] R (CGA TCG AGT GAT CCG GTG AAT TA) ITS2 F (GTG AAC CTT ATC ACT TAA AGG) ~300 Denaturation at 96 °C for 3 min; 30 cycles at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 1 min 30 s; extension at 72 °C for 7 min Duarte et al. [50] R (CAA CTC CTC CAC GCA ATC G) hsp70 Bvog751Fb (CCG TAT CCC CAA GAT CCA GC) ~170 Denaturation at 95 °C for 5 min; 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; and final extension of 72 °C for 5 min This study Bvog926Rb (AGA GGG GAG CAA CGT CCA A) F1 (CAT GAA GCA CTG GCC HTT CAA) ~740 Denaturation at 95 °C for 5 min; 36 cycles at 95 °C for 15 s, 58.5–60 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Soares et al. [1] R1a (GCN CKG CTG ATG GTG GTG TTG TA) F2a (GGA TCA ACA AYG GMA AGA AC) ~720 R2 (GBA GGT TGT TGT CCT TVG TCA T) cox1 F (GGA AGT GGW ACW GGW TGG AC) ~900 Denaturation at 95 °C for 1 min; 35 cycles at 95 °C for 15 s, 45–57 °C for 30 s, and 72 °C for 1 min; and extension at 72 °C for 10 min Schreeg et al. [42] R (TTC GGT ATT GCA TGC CTT G) cox3 F (ACT GTC AGC TAA AAC GTA TC) ~700 Denaturation at 94 °C for 5 min; 40 cycles at 94 °C for 20 s, 55 °C for 30 s, and 68 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (ACA GGA TTA GAT ACC CTG G) cytb F (TTA GTG AAG GAA CTT GAC AGG T) ~900 Denaturation at 95 °C for 5 s; 35 cycles at 94 °C for 20 s, 58 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (CGG TTA ATC TTT CCT ATT CCT TAC G) University).
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis.
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi, and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: Beyond the known: Unraveling the existence of novel Trichinella genotypes (T14 and T15) in India and expanding the species complex
Article Snippet: A consensus sequence for each isolate was generated using BioEdit version 7.2.5 and subsequently submitted to the GenBank database, from which accession numbers were obtained ( ).
Article Title: Impact of Ozonated Water on the Fungal Colonies, Diversity and Fruit Quality of Grapevine in Northern Europe
Article Snippet: The sequences were edited using BioEdit version 7.2.5 [24].
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi , and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: PLOS Neglected Tropical Diseases
Article Snippet: A sequence identity 198 matrix was obtained with BioEdit version 7.2.5.
Countercurrent Chromatography:Article Title: Male-dependent resistance to Spiroplasma -induced cytoplasmic incompatibility
Article Snippet: Consensus sequences per Spiroplasma strain and gene were created with MEGA version 11 [ ] and BioEdit version 7.2.5 [ ] by manual analysis and editing.
Article Title: Helminth fauna of the monocled cobra (Naja kaouthia) from central Thailand: Community composition and taxonomic perspectives
Article Snippet: The obtained sequences were manually reviewed and edited using BioEdit version 7.2.5 (Hall, 1999).
Article Title: Are Babesia vogeli genotypes associated with Rhipicephalus sanguineus and Rhipicephalus linnaei distribution?
Article Snippet: Forward and reverse sequences were aligned and trimmed (error probability = 0.05) to generate consensus sequences using BioEdit version 7.2.5 (North Carolina State a Internal primers used for sequencing b Primers used on the screening PCR 18S rRNA Piro 0F (GCC AGT AGT CAT ATG CTT GTC TCA) ~1500 Denaturation at 95 °C for 5 min; 35 cycles at 94 °C for 1 min 30 s, 58 °C for 1 min, and 72 °C for 2 min; and extension at 72 °C for 10 min Kawabuchi et al. [47], Soares et al. [1], Mongruel et al. [48]Piro 6R (CTC CTT CCT YTA AGT GAT AAG GTT CAC) Piro 5.5Ra (CCT YTA AGT GAT AAG GTT CAC AAA ACT T) Bab 2fa (CCG TGC TAA TTG TAG GGC TAA TAC A) Bab 2f2a (CTT TGA GAA ATT AGA GTG TTT) Bab 2ra (TTC CTT AGA TGT GGT AGC CGT TTC) ITS1 F (GCT GCG TCC TTC ATC GTT GTG) ~300 Denaturation at 94 °C for 2 min; 30 cycles at 94 °C for 30 s, 60–66 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Bostrom et al. [49] R (CGA TCG AGT GAT CCG GTG AAT TA) ITS2 F (GTG AAC CTT ATC ACT TAA AGG) ~300 Denaturation at 96 °C for 3 min; 30 cycles at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 1 min 30 s; extension at 72 °C for 7 min Duarte et al. [50] R (CAA CTC CTC CAC GCA ATC G) hsp70 Bvog751Fb (CCG TAT CCC CAA GAT CCA GC) ~170 Denaturation at 95 °C for 5 min; 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; and final extension of 72 °C for 5 min This study Bvog926Rb (AGA GGG GAG CAA CGT CCA A) F1 (CAT GAA GCA CTG GCC HTT CAA) ~740 Denaturation at 95 °C for 5 min; 36 cycles at 95 °C for 15 s, 58.5–60 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Soares et al. [1] R1a (GCN CKG CTG ATG GTG GTG TTG TA) F2a (GGA TCA ACA AYG GMA AGA AC) ~720 R2 (GBA GGT TGT TGT CCT TVG TCA T) cox1 F (GGA AGT GGW ACW GGW TGG AC) ~900 Denaturation at 95 °C for 1 min; 35 cycles at 95 °C for 15 s, 45–57 °C for 30 s, and 72 °C for 1 min; and extension at 72 °C for 10 min Schreeg et al. [42] R (TTC GGT ATT GCA TGC CTT G) cox3 F (ACT GTC AGC TAA AAC GTA TC) ~700 Denaturation at 94 °C for 5 min; 40 cycles at 94 °C for 20 s, 55 °C for 30 s, and 68 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (ACA GGA TTA GAT ACC CTG G) cytb F (TTA GTG AAG GAA CTT GAC AGG T) ~900 Denaturation at 95 °C for 5 s; 35 cycles at 94 °C for 20 s, 58 °C for 30 s, and 72 °C for 1 min 30 s; and extension at 72 °C for 7 min Schreeg et al. [42] R (CGG TTA ATC TTT CCT ATT CCT TAC G) University).
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis.
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi, and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: Beyond the known: Unraveling the existence of novel Trichinella genotypes (T14 and T15) in India and expanding the species complex
Article Snippet: A consensus sequence for each isolate was generated using BioEdit version 7.2.5 and subsequently submitted to the GenBank database, from which accession numbers were obtained ( ).
Article Title: Impact of Ozonated Water on the Fungal Colonies, Diversity and Fruit Quality of Grapevine in Northern Europe
Article Snippet: The sequences were edited using BioEdit version 7.2.5 [24].
Article Title: Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis
Article Snippet: Species-specific primers targeting the msp1 gene of P. knowlesi, P. cynomolgi , and P. inui were designed manually following multiple sequence alignment using BioEdit version 7.2.5.
Article Title: PLOS Neglected Tropical Diseases
Article Snippet: A sequence identity 198 matrix was obtained with BioEdit version 7.2.5.
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